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TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN
Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor
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Journal : BERITA BIOLOGI

 1 
VARIASI INTERSPESIFIK JULANG (AVES: BUCEROTIDAE) INDONESIA BERDASARKAN GEN CYTOCHROME-B DNA MITOKONDRIA Jarulis, Jarulis; Solihin, Dedy Duryadi; Mardiastuti, Ani; Prasetyo, Lilik Budi
BERITA BIOLOGI Vol 18, No 1 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i1.3714

Abstract

Genetic information of Indonesian hornbills (Julang) is still limited. We sequenced the cytochrome b gene of three hornbill species (Rhyticeros undulatus, R. plicatus, and Aceros cassidix) to explore their genetic variation, distance, and phylogenetic. Nine blood samples for Cyt b from three hornbill species were isolated with Dneasy® Blood and Tissue Kit base on Spin-Column Protocol, Qiagen. SNP, genetic distance, and phylogeny were calculated by MEGA 6.0 software. This study was found variations in the Cyt b gene sequence (849 bp) between three species Indonesian Julang. Rhyticeros undulatus had the most SNP compared to R. plicatus and Aceros cassidix. Substitution of synonyms is more common than substitution of synonyms and transition substitution frequency tends to be much higher than transversion substitution. The three species of Indonesian Julang were markedly separate with an average genetic distance of 5.25%. and separated with the outgroup species at a genetic distance of 9.43%. Cytochrome b sequence genes from these three hornbill species are novel for identifying and classifying hornbills, and could be applied to prevent illegal poaching and conservation management in Indonesia.    
KAJIAN KERAGAMAN GENETIK BURUNG KAKATUA TANIMBAR (CACATUA GOFFINI, FINSCH) MENGGUNAKAN PENCIRI "RAPD" Astuti, Dwi; Priyono, Siti N.; Julistiono, Heddy; Duryadi, Dedy
BERITA BIOLOGI Vol 4, No 2&3 (1998)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i2&3.1277

Abstract

The study was conducted to analyse genetic diversity of Goffini Finsch Cockatoo (Cacatua goffini Finsch) bird using RAPD (Random Amplified Polymorphic DNA) marker.PCR (Polymerase Chain Reaction) was performed on DNA samples extracted from 14 birds,using 18 random 10-merprimers and 2 random 12-merprimers. Fourteen out of 20 primers (70 %) successfully amplified DNAfragmens and 11 out of 14 primers (78,57 %) generated 1-2 specific alkies.The result clearly demonstrated that the RAPD marker allows for genetic diversity analyses of these birds in efficiently. Tree of relationship among 14 birds showed that there were two groups in the population ofGoffin 's Cockatoo.
VARIASI INTERSPESIFIK JULANG (AVES: BUCEROTIDAE) INDONESIA BERDASARKAN GEN CYTOCHROME-B DNA MITOKONDRIA Jarulis Jarulis; Dedy Duryadi Solihin; Ani Mardiastuti; Lilik Budi Prasetyo
BERITA BIOLOGI Vol 18, No 1 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i1.3714

Abstract

Genetic information of Indonesian hornbills (Julang) is still limited. We sequenced the cytochrome b gene of three hornbill species (Rhyticeros undulatus, R. plicatus, and Aceros cassidix) to explore their genetic variation, distance, and phylogenetic. Nine blood samples for Cyt b from three hornbill species were isolated with Dneasy® Blood and Tissue Kit base on Spin-Column Protocol, Qiagen. SNP, genetic distance, and phylogeny were calculated by MEGA 6.0 software. This study was found variations in the Cyt b gene sequence (849 bp) between three species Indonesian Julang. Rhyticeros undulatus had the most SNP compared to R. plicatus and Aceros cassidix. Substitution of synonyms is more common than substitution of synonyms and transition substitution frequency tends to be much higher than transversion substitution. The three species of Indonesian Julang were markedly separate with an average genetic distance of 5.25%. and separated with the outgroup species at a genetic distance of 9.43%. Cytochrome b sequence genes from these three hornbill species are novel for identifying and classifying hornbills, and could be applied to prevent illegal poaching and conservation management in Indonesia.    
KAJIAN KERAGAMAN GENETIK BURUNG KAKATUA TANIMBAR (Cacatua goffini, Finsch) MENGGUNAKAN PENCIRI "RAPD" Dwi Astuti; Siti N. Priyono; Heddy Julistiono; Dedy Duryadi
BERITA BIOLOGI Vol 4, No 2&3 (1998)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i2&3.1277

Abstract

The study was conducted to analyse genetic diversity of Goffini Finsch Cockatoo (Cacatua goffini Finsch) bird using RAPD (Random Amplified Polymorphic DNA) marker.PCR (Polymerase Chain Reaction) was performed on DNA samples extracted from 14 birds,using 18 random 10-merprimers and 2 random 12-merprimers. Fourteen out of 20 primers (70 %) successfully amplified DNAfragmens and 11 out of 14 primers (78,57 %) generated 1-2 specific alkies.The result clearly demonstrated that the RAPD marker allows for genetic diversity analyses of these birds in efficiently. Tree of relationship among 14 birds showed that there were two groups in the population ofGoffin 's Cockatoo.
Co-Authors Abdul Rahman Singkam Achmad - Taher ACHMAD FARAJALLAH Achmad Machmud Achmad Machmud Thohari Achmad Taher Achmad Taher Achmad, Taher Agus Nuryanto Agus Wahyana Anggara Agus Wahyana Anggara Agus Wahyana Anggara, Agus Wahyana Alam Putra Persada Ani Mardiastuti Ani Mardiastuti Ani Suryani Antonius Suwanto dan Meity S. Sinaga . Budi Tjahjono Andi Khaeruni R Any Aryani Ardi Kapahang Arief Boediono Arzyana Sunkar Bahiyah Bahiyah Bambang Purwantara Cece Sumantri CECILIA ANNA SEUMAHU CHRISTIAN HANSJOACHIM SCHULZE D.D. Sastraatmadja DAMAYANTI BUCHORI Dedi . Soedharma Dedi Soedharma DEWI APRI ASTUTI Dewi Elfidasari Dewi Malia Prawiradilaga Dewi Malia Prawiradilaga, Dewi Malia DIAH ISKANDRIATI Diah Iskandriati DIETMAR BLOHM Dodi Nandika Dondin Sajuthi Dwi ASTUTI Dwi Astuti Dwi Sendi Priyono DYAH PERWITASARI -FARAJALLAH Epa Paujiah, Epa EVY AYU ARIDA Evy Ayu Arida Fahma Wijayanti FAHRI FAHRUDIN Findra, Muhammad Nur FUNGKEY HOETAMA Gita Kusuma Rahayu Hadi Allikodra Hadi S Alikodra Hadi S Alikodra HADI SUKADI ALIKODRA HAJRIAL ASWIDINNOOR Handayani Handayani Hari Prayogo Harini Nurcahya Mariandayani Heddy Julistiono HEDDY JULISTIONO Hermanu Triwidodo I Gusti Agung Arta Putra I WAYAN SUANA Ibnu Maryanto Iman Rusmana Irma Shita Arlyza Irzaman, Irzaman Isdradjad Setyobudiandi Ismayati Afifah Jamhari Jamhari Jarulis Jarulis Jarulis Jarulis Jarulis Jarulis Jito Jito Jito Sugardjito Joko Pamungkas Jusmaldi Jusmaldi Kadarwan Soewardi Khustina, Yenny Chusna Khustina, Yenny Chusna Kunio Watanabe Lia Aseptin Murdini Lilik Budi Lilik Budi Prasetyo Lilik Budi Prasetyo Lucia Johana Lambey M F Rahardjo M. F. Rahardjo M. Zairin Junior MARIA BINTANG MF Rahardjo Mustafa Sabri Nastiti Kusumorini NEVIATY PUTRI ZAMANI Niken Subekti Niken TM Pratiwi Nurlisa Butet, Nurlisa Prasetyo Prasetyo Priyono, Siti N. Retno Damayanti Soejoedono RICHARD F GRANT Ridwan Affandi Ridwan Affandi Ridwan Affandi Ridwan Affandi Ridwan Affandi Ridwan Affandi Ridwan Affandi RIDWAN AFFANDI Rini Widayanti Robba Fahrisy Darus ROEDHY POERWANTO Roedy Poerwanto Roedy Poerwanto Roni Koneri Ronny Rachman Noor Roza Elvyra Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Rudi Afnan Rudi Tarumingkeng Rudy C Tarumingkeng Safrida Safrida Saroyo Saroyo SATA YOSHIDA SRIE RAHAYU SATRIYAS ILYAS SELA SEPTIMA MARIYA SILMI MARIYA Siti N. Priyono SJAFRIDA MANUWOTO Soaloon Sinaga Soaloon Sinaga Sobir Sobir Sobir Sobir Sobir Sobir Solihin Solihin Sri Catur Setyawatiningsih Sri Catur Setyawatiningsih sri murtini . SRI NINGSIH Sri Sulandari Sri Supraptini Mansjoer Subyakto Subyakto Sugardjito Sugardjito Suharsono Suharsono SULISTIYANI SULISTIYANI Sulistiyani Sulistiyani SULISTIYANI SULISTIYANI Sulistiyani, Sulistiyani Surjono Surjokusumo Syaiful Anwar Syamsul Bachry Tedjo Sukmono Thohari Thohari Tike Sartika Tri Haryoko Tri Haryoko, Tri UUS SAEPULOH UUS SAEPULOH Wasmen Manalu Yuli Wahyu Tri Mulyani YULIN LESTARI Yuni Cahya Endrawati Yus Rusila Noor Yusnarti Yus